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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PCOTH Polyclonal antibody (24564-1-AP)

    IF Staining of PC-3 using 24564-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PCOTH antibody (24564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PCOTH Polyclonal antibody

PCOTH Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 24564-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

PCOTH, prostate collagen triple helix, Protein PCOTH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24564-1-AP targets PCOTH in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCOTH fusion protein Ag20092 相同性解析による交差性が予測される生物種
Full Name prostate collagen triple helix
Calculated molecular weight 107 aa, 11 kDa
GenBank accession numberBC142999
Gene Symbol PCOTH
Gene ID (NCBI) 542767
RRIDAB_2918084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCOTH may be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.

Protocols

Product Specific Protocols
IHC protocol for PCOTH antibody 24564-1-APDownload protocol
IF protocol for PCOTH antibody 24564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols