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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PCP4L1 Recombinant antibody (83093-2-RR)

    IF Staining of Caco-2 using 83093-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCP4L1 antibody (83093-2-RR, Clone: 230203G12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Caco-2 cells using PCP4L1 Recombinant antibody (83093-2-RR)

    FC experiment of Caco-2 using 83093-2-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug Anti-Human PCP4L1 (83093-2-RR, Clone:230203G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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PCP4L1 Recombinant antibody

PCP4L1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83093-2-RR
Clone No.230203G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Purkinje cell protein 4-like protein 1, Purkinje cell protein 4 like 1, PCP4-like protein 1, PCP4 like protein 1, IQM1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83093-2-RR targets PCP4L1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCP4L1 fusion protein Ag31230 相同性解析による交差性が予測される生物種
Full Name Purkinje cell protein 4 like 1
Calculated molecular weight 68 aa, 7 kDa
GenBank accession numberBC028905
Gene Symbol PCP4L1
Gene ID (NCBI) 654790
RRIDAB_3670807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA6NKN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

Protocols

Product Specific Protocols
IF protocol for PCP4L1 antibody 83093-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols