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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCSK1 Polyclonal antibody

PCSK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28219-1-AP

Synonyms

BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCSK1 Polyclonal antibody (28219-1-AP)

    WB analysis using 28219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of human pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of human pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of mouse pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of mouse pancreas using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of mouse brain using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCSK1 Polyclonal antibody (28219-1-AP)

    IHC staining of mouse brain using 28219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28219-1-AP targets PCSK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK1 fusion protein Ag28203 相同性解析による交差性が予測される生物種
Full Name proprotein convertase subtilisin/kexin type 1
Calculated molecular weight 753 aa, 84 kDa
Observed molecular weight66 kDa
GenBank accession numberBC130295
Gene symbol PCSK1
Gene ID (NCBI) 5122
RRIDAB_2881093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PCSK1 antibody 28219-1-APDownload protocol
IHC protocol for PCSK1 antibody 28219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Nutr Food Res

The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation

Authors - Mengcheng Jin
View Article
humanIHC

Rapid Commun Mass Spectrom

Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm

Authors - Meng Zhang
View Article