Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
10553-1-AP targets PCSK2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCSK2 fusion protein Ag0852 相同性解析による交差性が予測される生物種 |
| Full Name | proprotein convertase subtilisin/kexin type 2 |
| Calculated molecular weight | 70.5 kDa |
| Observed molecular weight | 65-75 kDa |
| GenBank accession number | BC005815 |
| Gene Symbol | PCSK2 |
| Gene ID (NCBI) | 5126 |
| RRID | AB_2299124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCSK2 antibody 10553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells. | ||
Function (Oxf) ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell | ||
Front Mol Biosci Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease | ||
iScience Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells |