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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCSK4 Polyclonal antibody

PCSK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15106-1-AP

Synonyms

PC4, PCSK4, Proprotein convertase 4, SPC5

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    WB analysis of human testis using 15106-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15106-1-AP (PCSK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    WB analysis of human placenta using 15106-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15106-1-AP (PCSK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    IHC staining of human placenta using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    IHC staining of human placenta using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    IHC staining of human testis using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PCSK4 Polyclonal antibody (15106-1-AP)

    IHC staining of human testis using 15106-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman testis tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15106-1-AP targets PCSK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK4 fusion protein Ag4007 相同性解析による交差性が予測される生物種
Full Name proprotein convertase subtilisin/kexin type 4
Calculated molecular weight 83 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC036354
Gene symbol PCSK4
Gene ID (NCBI) 54760
RRIDAB_10644152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proprotein convertase subtilysin/Kexin Type4 (PCSK4) is a protein convertase enzymes in spermatozoa acrosome membrane, it has an important role in the acrosome reaction through endoproteolytic process that converts the precursor proteins into bioactive protein as a necessary substance to penetrate on the zona pellucida (PMID: 16040806 ). The molecular mass of pro-PCSK4 is 72kDa, and mature PCSK4 is 54 kDa (PMID: 16040806, 19109312).

Protocols

Product Specific Protocols
WB protocol for PCSK4 antibody 15106-1-APDownload protocol
IHC protocol for PCSK4 antibody 15106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols