PCSK7 Polyclonal antibody
PCSK7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12044-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
12044-1-AP targets PCSK7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCSK7 fusion protein Ag2674 相同性解析による交差性が予測される生物種 |
| Full Name | proprotein convertase subtilisin/kexin type 7 |
| Calculated molecular weight | 785 aa, 86 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC010696 |
| Gene symbol | PCSK7 |
| Gene ID (NCBI) | 9159 |
| RRID | AB_2877820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCSK7(Proprotein convertase subtilisin/kexin type 7) is also named as LPC, PC7, PC8, SPC7 and belongs to the peptidase S8 family. It has ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif. The full length protein has 4 glycosylation sites and it can be N-glycosylated.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCSK7 antibody 12044-1-AP | Download protocol |
| IHC protocol for PCSK7 antibody 12044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Therapeutic potential of the Proprotein Convertase Subtilisin/Kexin family in vascular disease |
