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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PCSK9 Recombinant antibody (84172-4-RR)

    WB analysis of HepG2 using 84172-4-RR (same clone as 84172-4-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84172-4-RR (PCSK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84172-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01071-4

    Sandwich ELISA standard curve of MP01071-4

    Sandwich ELISA standard curve of MP01071-4, Human PCSK9 Recombinant Matched Antibody Pair - PBS only. 84172-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0362. 84172-6-PBS was HRP conjugated as the detection antibody. Range: 195-6250 pg/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    Plasma of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 206.0 ng/mL with a range of ND-514.5 ng/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human PCSK9, and measured 81.6 ng/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    The mean PCSK9 concentration was determined to be 6.6 ng/mL in HepG2 cell extract based on a 2.0 mg/mL extract load.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    Serum of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 120.8 ng/mL with a range of ND-421.1 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCSK9 Recombinant antibody (84172-4-RR)

    IF Staining of HepG2 using 84172-4-RR (same clone as 84172-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCSK9 antibody (84172-4-RR, Clone: 241395G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84172-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PCSK9 Recombinant antibody (84172-4-RR)

    FC experiment of HeLa using 84172-4-RR (same clone as 84172-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (84172-4-RR, Clone:241395G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84172-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01071-3

    Cytometric bead array standard curve of MP01071-3

    Cytometric bead array standard curve of MP01071-3, PCSK9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84172-4-PBS. Detection antibody: 84172-3-PBS. Standard: Eg0362. Range: 1.563-100 ng/mL.

  • Affinity and Kinetic Characterization of 84172-4-RR

    Affinity and Kinetic Characterization of 84172-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84172-4-RR against Human PCSK9 were performed. The affinity constant is 0.80 nM.

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PCSK9 Recombinant antibody, PBS Only (Capture)

PCSK9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84172-4-PBS
Clone No.241395G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241395G1, EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84172-4-PBS targets PCSK9 as part of a matched antibody pair:

MP01071-3: 84172-4-PBS capture and 84172-3-PBS detection (validated in Cytometric bead array)

MP01071-4: 84172-4-PBS capture and 84172-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name proprotein convertase subtilisin/kexin type 9
Calculated molecular weight74 kDa
Observed molecular weight 72-78 kDa, 62 kDa
GenBank accession numberNM_174936.4
Gene Symbol PCSK9
Gene ID (NCBI) 255738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NBP7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels. (PMID: 30522786, PMID: 22493497)