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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCTAIRE3 Polyclonal antibody

PCTAIRE3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12207-1-AP

Synonyms

PCTK3, PCTAIRE-motif protein kinase 3, PCTAIRE, EC:2.7.11.22, Cyclin-dependent kinase 18

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PCTAIRE3 Polyclonal antibody (12207-1-AP)

    WB analysis of HEK-293 using 12207-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PCTAIRE3 Polyclonal antibody (12207-1-AP)

    WB analysis of HEK-293 using 12207-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PCTAIRE3 Polyclonal antibody (12207-1-AP)

    WB analysis of mouse brain using 12207-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PCTAIRE3 Polyclonal antibody (12207-1-AP)

    IHC staining of human ovary cancer using 12207-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PCTAIRE3 Polyclonal antibody (12207-1-AP)

    IF Staining of HEK-293 using 12207-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12207-1-AP targets PCTAIRE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCTAIRE3 fusion protein Ag2851 相同性解析による交差性が予測される生物種
Full Name PCTAIRE protein kinase 3
Calculated molecular weight 472 aa, 54 kDa
Observed molecular weight 54 kDa, 58 kDa
GenBank accession numberBC011526
Gene symbol PCTAIRE3
Gene ID (NCBI) 5129
RRIDAB_906375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCTAIRE3 (PCTAIRE-motif protein kinase 3), also named CDK18 or PCTK3, is a member of PCTAIRE subfamily. The PCTAIRE kinase (PCTK) subfamily of CDKs includes three members, PCTK1/CDK16, PCTK2/CDK17, and PCTK3/CDK18, which contain the PCTAIRE sequence instead of the PSTAIRE sequence (PMID: 24831015). PCTK3 has three isoforms with the molecular mass of 54-58kD.

Protocols

Product Specific Protocols
WB protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IHC protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
IF protocol for PCTAIRE3 antibody 12207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols