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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCYT2 Monoclonal antibody (68355-1-Ig)

    WB analysis using 68355-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68355-1-Ig (PCYT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCYT2 Monoclonal antibody (68355-1-Ig)

    IF Staining of HepG2 using 68355-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCYT2 antibody (68355-1-Ig, Clone: 2F7G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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PCYT2 Monoclonal antibody

PCYT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68355-1-Ig
Clone No.2F7G1

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

ET, PCYT2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68355-1-Ig targets PCYT2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PCYT2 fusion protein Ag6604 相同性解析による交差性が予測される生物種
Full Name phosphate cytidylyltransferase 2, ethanolamine
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC000351
Gene Symbol PCYT2
Gene ID (NCBI) 5833
RRIDAB_3085077
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99447
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

Protocols

Product Specific Protocols
WB protocol for PCYT2 antibody 68355-1-IgDownload protocol
IF protocol for PCYT2 antibody 68355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols