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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse PD-1/CD279 Recombinant antibody, PBS Only (Capture)

PD-1/CD279 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240390D5

Cat no : 83516-4-PBS

Synonyms

CD279, PD-1, PD1, Pdc1, PD 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00496-4

    Sandwich ELISA standard curve of MP00496-4

    Sandwich ELISA standard curve of MP00496-4, Mouse PD-1 Monoclonal Matched Antibody Pair - PBS only. 83516-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0918. 83516-9-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Sample test of MP00496-4

    Sample test of MP00496-4

    The mean mouse PD-1 concentration was determined to be 14.08 ng/mL in EL-4 cell extract based on a 3.0 mg/mL extract load.

  • Sample test of MP00496-4

    Sample test of MP00496-4

    EL-4 was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL ofstreptomycin sulfate.The cellswere stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant wasremoved, assayed for mouse PD-1 and measured 812.8 pg/mL.

  • Cytometric bead array standard curve of MP00496-1

    Cytometric bead array standard curve of MP00496-1

    Cytometric bead array standard curve of MP00496-1, MOUSE PD-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83516-4-PBS. Detection antibody: 83516-5-PBS. Standard: Eg0918. Range: 0.156-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83516-4-PBS targets PD-1/CD279 as part of a matched antibody pair:

MP00496-1: 83516-4-PBS capture and 83516-5-PBS detection (validated in Cytometric bead array)

MP00496-4: 83516-4-PBS capture and 83516-9-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight32 kDa
GenBank accession numberNM_008798.2
Gene symbol PD-1
Gene ID (NCBI) 18566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.