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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recombinant Antibody, PBS Only

PD-1/CD279 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

EH12.2H7

Cat no : 65586-1-PBS

Synonyms

CD279, PD1, PD-1, PD 1, hPD-1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recom (65586-1-MR)

    FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. This data was developed using the same antibody clone with 65586-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recom (65586-1-MR)

    FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). 1x10^6 PHA treated human PBMCs were surface stained with Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 65586-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recom (65586-1-MR)

    FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) (left) or 0.25 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone: EH12.2H7) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. This data was developed using the same antibody clone with 65586-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65586-1-PBS targets PD-1/CD279 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
GenBank accession numberBC074740
Gene symbol PD-1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).