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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-L1 (B7-H1) (29E.2A3)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

29E.2A3

Cat no : 65081-1-Ig

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-L1 (B7-H1) (29E.2A3) (65081-1-Ig)

    FC experiment of human PBMCs using 65081-1-Ig

    1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG2b Isotype Control (blue). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-L1 (B7-H1) (29E.2A3) (65081-1-Ig)

    FC experiment of human PBMCs using 65081-1-Ig

    1X10^6 PHA treated (3d) human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (right), or Mouse IgG2b Isotype Control (left). 1X10^6 untreated human PBMCs were surface stained with 0.2 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone:29E.2A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (center). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65081-1-Ig targets PD-L1 (B7-H1) in FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Full length human PD-L1 相同性解析による交差性が予測される生物種
Full Name CD274 molecule
GenBank accession numberBC069381
Gene symbol PD-L1
Gene ID (NCBI) 29126
RRIDAB_2918384
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
FC protocol for PD-L1 (B7-H1) antibody 65081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Transl Res

Immune checkpoint activity exacerbate renal interstitial fibrosis progression by enhancing PD-L1 expression in renal tubular epithelial cells

Authors - Yuting Zhang
  • KD Validated
View Article