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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody

PD-L1/CD274 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240721H8

Cat no : 98062-1-RR

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR)

    FC experiment of human PBMCs using 98062-1-RR

    1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR)

    FC experiment of human PBMCs using 98062-1-RR

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98062-1-RR

    Affinity and Kinetic Characterization of 98062-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98062-1-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.558 nM.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98062-1-RR is PD-L1/CD274 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD274 molecule
Calculated molecular weight 290 aa, 33 kDa
GenBank accession numberBC074984
Gene symbol PD-L1
Gene ID (NCBI) 29126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Protocols

Product Specific Protocols
FC protocol for PD-L1/CD274 antibody 98062-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols