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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PD-L2/B7-DC Recombinant antibody, PBS Only (Detector)

PD-L2/B7-DC Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230514A2

Cat no : 83158-1-PBS

Synonyms

PDCD1LG2, PD-L2, CD273, Butyrophilin B7-DC, B7-DC

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00778-4

    Sandwich ELISA standard curve of MP00778-4

    Sandwich ELISA standard curve of MP00778-4, Human PD-L2/B7-DCMonoclonal Matched Antibody Pair - PBS only. 83158-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0507. 83158-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00778-4

    Sample test of MP00778-4

    Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

  • Sample test of MP00778-4

    Sample test of MP00778-4

    For the PBMC supernatant cultured for 4 days, the mean PD-L2/B7-DC concentration was determined to be 14.8 pg/mL in unstimulated PBMC supernatant, 58.7 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00778-4

    Sample test of MP00778-4

    Milk of eight individual healthy human donors was measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 394.5 pg/mL with a range of 206.2 - 1,153.2 pg/mL.

  • Sample test of MP00778-4

    Sample test of MP00778-4

    Serum of fifteen individual healthy human donors were measured. The human PD-L2/B7-DC concentration of detected samples was determined to be 13,665.12 pg/mL with a range of 7,054.02 - 18,074.46 pg/mL.

  • Cytometric bead array standard curve of MP00778-1

    Cytometric bead array standard curve of MP00778-1

    Cytometric bead array standard curve of MP00778-1, PD-L2/B7-DC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83158-3-PBS. Detection antibody: 83158-1-PBS. Standard: Eg0507. Range: 0.625-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83158-1-PBS targets PD-L2/B7-DC as part of a matched antibody pair:

MP00778-1: 83158-3-PBS capture and 83158-1-PBS detection (validated in Cytometric bead array)

MP00778-4: 83158-3-PBS capture and 83158-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1 ligand 2
Calculated molecular weight 273 aa, 31 kDa
GenBank accession numberBC074766
Gene symbol PD-L2
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.