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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PDAP1 Polyclonal antibody (15081-1-AP)

    WB analysis of MCF-7 using 15081-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15081-1-AP (PDAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PDAP1 Polyclonal antibody (15081-1-AP)

    WB analysis of MCF-7 using 15081-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15081-1-AP (PDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PDAP1 Polyclonal antibody (15081-1-AP)

    IP experiment of MCF-7 using 15081-1-AP

    IP result of anti-PDAP1 (IP:15081-1-AP, 4ug; Detection:15081-1-AP 1:1000) with MCF-7 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDAP1 Polyclonal antibody (15081-1-AP)

    IHC staining of human breast cancer using 15081-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDAP1 Polyclonal antibody (15081-1-AP)

    IHC staining of human breast cancer using 15081-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PDAP1 Polyclonal antibody (15081-1-AP)

    IF Staining of MCF-7 using 15081-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDAP1 antibody (15081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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PDAP1 Polyclonal antibody

PDAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15081-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15081-1-AP targets PDAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDAP1 fusion protein Ag7155 相同性解析による交差性が予測される生物種
Full Name PDGFA associated protein 1
Calculated molecular weight 21 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC000684
Gene Symbol PDAP1
Gene ID (NCBI) 11333
RRIDAB_2159306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDAP1 antibody 15081-1-APDownload protocol
IHC protocol for PDAP1 antibody 15081-1-APDownload protocol
IF protocol for PDAP1 antibody 15081-1-APDownload protocol
IP protocol for PDAP1 antibody 15081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proteomics Clin Appl

SILAC-based quantitative proteomic analysis of gastric cancer secretome.

Authors - Marimuthu Arivusudar A
View Article
humanWB

Cancer Sci

Pancreatic ductal adenocarcinoma cells regulated the gemcitabine-resistance function of CAFs by LINC00460

Authors - Xiao‐Xu Zhu
View Article
human

Heliyon

Single-cell RNA-Seq reveals the heterogeneity of fibroblasts within the tympanojugular paraganglioma microenvironment

Authors - Shengming Wang
View Article
human,mouseWB,CoIP

Sci Adv

Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses

Authors - Takayoshi Shirasaki
View Article