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  • Featured Product
  • KD/KO Validated

CCM3/PDCD10 Monoclonal antibody

CCM3/PDCD10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E5A8

Cat no : 66440-1-Ig

Synonyms

CCM3, PDCD10, programmed cell death 10, TFAR15

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    WB analysis of U-251 using 66440-1-Ig

    WB result of CCM3/PDCD10 antibody (66440-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDCD10 transfected U-251 cells.

  • Western Blot (WB) analysis of Jurkat cells using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    WB analysis of Jurkat using 66440-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    WB analysis of Ramos using 66440-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    WB analysis of human peripheral blood platelets using 66440-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    IHC staining of human colon cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    IHC staining of human colon cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    IHC staining of human liver cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCM3/PDCD10 Monoclonal antibody (66440-1-Ig)

    IHC staining of human liver cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, human peripheral blood platelets, Ramos cells, U-251 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66440-1-Ig targets CCM3/PDCD10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CCM3/PDCD10 fusion protein Ag0348 相同性解析による交差性が予測される生物種
Full Name programmed cell death 10
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC002506
Gene symbol PDCD10
Gene ID (NCBI) 11235
RRIDAB_2881810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development.

Protocols

Product Specific Protocols
WB protocol for CCM3/PDCD10 antibody 66440-1-IgDownload protocol
IHC protocol for CCM3/PDCD10 antibody 66440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols