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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PD-L2/CD273 Polyclonal antibody

PD-L2/CD273 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27406-1-AP

Synonyms

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, PD-1 ligand 2, CD273

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDCD1LG2/PD-L2 Polyclonal antibody (27406-1-AP)

    WB analysis using 27406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PD-L2/CD273 Polyclonal antibody (27406-1-AP)

    IF Staining of NIH/3T3 using 27406-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inNIH/3T3 cells, Raji cells, mouse heart tissue, rat heart tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27406-1-AP targets PD-L2/CD273 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PD-L2/CD273 fusion protein Ag26384 相同性解析による交差性が予測される生物種
Full Name programmed cell death 1 ligand 2
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC074766
Gene symbol PD-L2
Gene ID (NCBI) 80380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).

Protocols

Product Specific Protocols
WB protocol for PD-L2/CD273 antibody 27406-1-APDownload protocol
IF protocol for PD-L2/CD273 antibody 27406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols