PDCD4 Polyclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.