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KD/KO Validated

PDCD6 Polyclonal antibody

Name: PDCD6 Polyclonal antibody
Brand: Proteintech
SKU: 12303-1-AP

PDCD6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12303-1-AP

Synonyms

programmed cell death 6, PEF1B, Apoptosis-linked gene 2 protein homolog, ALG-2, ALG2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 12303-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12303-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12303-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.
IP experiment of HeLa using 12303-1-AP
IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.
IHC staining of human lung cancer using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human stomach using 12303-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 12303-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 16 publications below
IHC	See 1 publications below
IF	See 7 publications below
IP	See 1 publications below

Product Information

12303-1-AP targets PDCD6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDCD6 fusion protein Ag2949 相同性解析による交差性が予測される生物種
Full Name	programmed cell death 6
Calculated molecular weight	191 aa, 22 kDa
Observed molecular weight	15-22 kDa
GenBank accession number	BC012384
Gene symbol	PDCD6
Gene ID (NCBI)	10016
RRID	AB_2162459
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PDCD6 antibody 12303-1-AP	Download protocol
IHC protocol for PDCD6 antibody 12303-1-AP	Download protocol
IP protocol for PDCD6 antibody 12303-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Structural basis for dimerization quality control. Authors - Elijah L Mena View Article
human	IF	Science Triggered recruitment of ESCRT machinery promotes endolysosomal repair. Authors - Michael L Skowyra View Article
human	WB,IF	Cell Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Authors - Colleen A McGourty KD Validated View Article
human	WB	Mol Cell Co-adaptor driven assembly of a CUL3 E3 ligase complex. Authors - David Akopian View Article
human	WB,IF	Cell Rep Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion. Authors - Johannes Westman KD Validated View Article
human	IF	J Cell Biol Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters Authors - Femmy C Stempels View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDCD6 Polyclonal antibody

PDCD6 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 12303-1-AP

WB analysis of Jurkat using 12303-1-AP

WB analysis using 12303-1-AP

IP experiment of HeLa using 12303-1-AP

IHC staining of human lung cancer using 12303-1-AP

IHC staining of human lung cancer using 12303-1-AP

IHC staining of human stomach using 12303-1-AP

IHC staining of human liver cancer using 12303-1-AP

IHC staining of human liver cancer using 12303-1-AP

IHC staining of human stomach using 12303-1-AP

IHC staining of human stomach using 12303-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Structural basis for dimerization quality control.

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Co-adaptor driven assembly of a CUL3 E3 ligase complex.

Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters