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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDCL Polyclonal antibody

PDCL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16057-1-AP

Synonyms

PDCL, PhLP, phosducin like, Phosducin like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDCL Polyclonal antibody (16057-1-AP)

    WB analysis using 16057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using PDCL Polyclonal antibody (16057-1-AP)

    WB analysis of NCI-H1299 using 16057-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDCL Polyclonal antibody (16057-1-AP)

    IHC staining of human placenta using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDCL Polyclonal antibody (16057-1-AP)

    IHC staining of human placenta using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDCL Polyclonal antibody (16057-1-AP)

    IHC staining of mouse brain using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16057-1-AP (PDCL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, NCI-H1299 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16057-1-AP targets PDCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCL fusion protein Ag9029 相同性解析による交差性が予測される生物種
Full Name phosducin-like
Calculated molecular weight 301 aa, 34 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC017227
Gene symbol PDCL
Gene ID (NCBI) 5082
RRIDAB_2236943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDCL antibody 16057-1-APDownload protocol
IHC protocol for PDCL antibody 16057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Physiol (Oxf)

ATP6V1A is required for synaptic rearrangements and plasticity in murine hippocampal neurons

Authors - Alessandro Esposito
View Article