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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDE10A Recombinant antibody

PDE10A Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242357E12

Cat no : 84869-1-RR

Synonyms

phosphodiesterase 10A, HSPDE10A, EC:3.1.4.17, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 242357E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDE10A Recombinant antibody (84869-1-RR)

    WB analysis using 84869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84869-1-RR (PDE10A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84869-1-RR

    Affinity and Kinetic Characterization of 84869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84869-1-RR against Human PDE10A were performed. The affinity constant is 3.37 nM.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84869-1-RR targets PDE10A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDE10A fusion protein Ag12771 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 10A
Calculated molecular weight 779 aa, 88 kDa
Observed molecular weight80 kDa
GenBank accession numberBC104860
Gene symbol PDE10A
Gene ID (NCBI) 10846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).

Protocols

Product Specific Protocols
WB protocol for PDE10A antibody 84869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols