PDE12 Polyclonal antibody, PBS Only

human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.