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  • KD/KO Validated

PDE1A Polyclonal antibody

PDE1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12442-2-AP

Synonyms

61 kDa Cam PDE, Cam PDE 1A, hCam 1, HCAM1, HSPDE1A, PDE1A


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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12442-2-AP targets PDE1A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE1A fusion protein Ag3157 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 1A, calmodulin-dependent
Calculated molecular weight 545 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC022480
Gene symbol PDE1A
Gene ID (NCBI) 5136
RRIDAB_2162893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).

Protocols

Product Specific Protocols
WB protocol for PDE1A antibody 12442-2-APDownload protocol
IHC protocol for PDE1A antibody 12442-2-APDownload protocol
IP protocol for PDE1A antibody 12442-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biol Reprod

Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis.

Authors - Jeremy R Egbert
mouseWB

Biol Reprod

Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†.

Authors - Jeremy R Egbert
mouseWB

PLoS One

Generation and phenotypic characterization of Pde1a mutant mice.

Authors - Xiaofang Wang
  • KO Validated

Cell Rep

Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development.

Authors - Ashley G Anderson
humanIHC

Front Genet

Transcriptome profiling of intact bowel wall reveals that PDE1A and SEMA3D are possible markers with roles in enteric smooth muscle apoptosis, proliferative disorders, and dysautonomia in Crohn's disease

Authors - Yun Yang