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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDE1B Polyclonal antibody (13121-1-AP)

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDE1B Polyclonal antibody (13121-1-AP)

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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PDE1B Polyclonal antibody

PDE1B Polyclonal Antibody for WB, ELISA
Cat No. 13121-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13121-1-AP targets PDE1B in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE1B fusion protein Ag3817 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 1B, calmodulin-dependent
Calculated molecular weight 536 aa, 61 kDa
Observed molecular weight61 kDa
GenBank accession numberBC032226
Gene Symbol PDE1B
Gene ID (NCBI) 5153
RRIDAB_3085407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01064
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE1B, also known as PDES1B, belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. PDE1B degrades cAMP and cGMP and regulates various important physiological processes such as proliferation and lipogenesis (PMID: 21960012). PDE1B is enriched in brain tissue. PDE1B gene consists of two isoforms, PDE1B1 (encoding 61 kDa protein with 536 amino acid residues) and PDE1B2 (encoding 59 kDa protein containing 516 amino acid residues).

Protocols

Product Specific Protocols
WB protocol for PDE1B antibody 13121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols