Validation Data Gallery
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Tested Applications
| Positive WB detected in | human heart tissue |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
13785-1-AP targets PDE1C in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDE1C fusion protein Ag4798 相同性解析による交差性が予測される生物種 |
| Full Name | phosphodiesterase 1C, calmodulin-dependent 70kDa |
| Calculated molecular weight | 634 aa, 72 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC022525 |
| Gene Symbol | PDE1C |
| Gene ID (NCBI) | 5137 |
| RRID | AB_2268145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14123 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C, is calmodulin-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex. Expression of PDE1C may be an important component in signal transduction pathways mediated by calcium that permit cell cycle traverse and proliferation(PMID:9366577). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE1C antibody 13785-1-AP | Download protocol |
| IP protocol for PDE1C antibody 13785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
Free Radic Biol Med Poly (I:C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries. |