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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using PDE3B Polyclonal antibody (25290-1-AP)

    WB analysis of HL-60 using 25290-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDE3B Polyclonal antibody (25290-1-AP)

    WB analysis of HeLa using 25290-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PDE3B Polyclonal antibody (25290-1-AP)

    IP experiment of HeLa using 25290-1-AP

    IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

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PDE3B Polyclonal antibody

PDE3B Polyclonal Antibody for WB, IP, ELISA
Cat No. 25290-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

EC:3.1.4.17, Cyclic GMP-inhibited phosphodiesterase B, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, cGIPDE1, CGI-PDE B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25290-1-AP targets PDE3B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE3B fusion protein Ag18064 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 3B, cGMP-inhibited
Calculated molecular weight 1112 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC136565
Gene Symbol PDE3B
Gene ID (NCBI) 5140
RRIDAB_2880010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13370
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDE3B antibody 25290-1-APDownload protocol
IP protocol for PDE3B antibody 25290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Mol Sci

Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions

Authors - Ning Liu
View Article