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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDE4D Monoclonal antibody (67062-1-Ig)

    WB analysis using 67062-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using PDE4D Monoclonal antibody (67062-1-Ig)

    WB analysis of pig cerebellum using 67062-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PDE4D Monoclonal antibody (67062-1-Ig)

    WB analysis of rat brain using 67062-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using PDE4D Monoclonal antibody (67062-1-Ig)

    WB analysis of rat cerebellum using 67062-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PDE4D Monoclonal antibody (67062-1-Ig)

    WB analysis of mouse brain using 67062-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PDE4D Monoclonal antibody (67062-1-Ig)

    IF Staining of A431 using 67062-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDE4D antibody (67062-1-Ig, Clone: 1C4B9 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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PDE4D Monoclonal antibody

PDE4D Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67062-1-Ig
Clone No.1C4B9
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67062-1-Ig targets PDE4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDE4D fusion protein Ag8302 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
Calculated molecular weight 809 aa, 91 kDa
Observed molecular weight 91-110 kDa, 66-84 kDa
GenBank accession numberBC036319
Gene Symbol PDE4D
Gene ID (NCBI) 5144
RRIDAB_2882372
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ08499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).

Protocols

Product Specific Protocols
WB protocol for PDE4D antibody 67062-1-IgDownload protocol
IF protocol for PDE4D antibody 67062-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun
View Article