PDE4D Monoclonal antibody
PDE4D Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C4B9
Cat no : 67062-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67062-1-Ig targets PDE4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PDE4D fusion protein Ag8302 相同性解析による交差性が予測される生物種 |
| Full Name | phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) |
| Calculated molecular weight | 809 aa, 91 kDa |
| Observed molecular weight | 91-110 kDa, 66-84 kDa |
| GenBank accession number | BC036319 |
| Gene symbol | PDE4D |
| Gene ID (NCBI) | 5144 |
| RRID | AB_2882372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE4D antibody 67062-1-Ig | Download protocol |
| IF protocol for PDE4D antibody 67062-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Med Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Angie (Verified Customer) (06-06-2023) | Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 67062-1-Ig (mouse anti-PDE4D antibody) at dilution of 1:10 000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.
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