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PDE6D Recombinant antibody

PDE6D Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230546H1

Cat no : 83503-1-RR

Synonyms

PDED, 230546H1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDE6D Recombinant antibody (83503-1-RR)

    WB analysis using 83503-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83503-1-RR (PDE6D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDE6D Recombinant antibody (83503-1-RR)

    WB analysis of HeLa using 83503-1-RR

    WB result of PDE6D antibody (83503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE6D transfected HeLa cells.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, T-47D cells, mouse brain tissue, rat brain tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83503-1-RR is PDE6D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDE6D fusion protein Ag29563 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 6D, cGMP-specific, rod, delta
Calculated molecular weight 17 KDa
Observed molecular weight 14-17 kDa
GenBank accession numberNM_002601
Gene symbol PDE6D
Gene ID (NCBI) 5147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Protocols

Product Specific Protocols
WB protocol for PDE6D antibody 83503-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols