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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PDE7A Polyclonal antibody (26277-1-AP)

    WB analysis of Jurkat using 26277-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26277-1-AP (PDE7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PDE7A Polyclonal antibody (26277-1-AP)

    WB analysis of K-562 using 26277-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26277-1-AP (PDE7A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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PDE7A Polyclonal antibody

PDE7A Polyclonal Antibody for WB, ELISA
Cat No. 26277-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

HCP1, PDE7, PDE7A, phosphodiesterase 7A, TM22

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26277-1-AP targets PDE7A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE7A fusion protein Ag24334 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 7A
Calculated molecular weight 482 aa, 56 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC126360
Gene Symbol PDE7A
Gene ID (NCBI) 5150
RRIDAB_2880458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13946
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDE7A antibody 26277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Phosphodiesterase 7 Regulation in Cellular and Rodent Models of Parkinson's Disease.

Authors - Jose A Morales-Garcia
View Article