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PDE7B Polyclonal antibody
PDE7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17771-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
17771-1-AP targets PDE7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDE7B fusion protein Ag12184 相同性解析による交差性が予測される生物種 |
| Full Name | phosphodiesterase 7B |
| Calculated molecular weight | 450 aa, 52 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC075082 |
| Gene symbol | PDE7B |
| Gene ID (NCBI) | 27115 |
| RRID | AB_2268032 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDE7B(cAMP-specific 3',5'-cyclic phosphodiesterase 7B) belongs to the cyclic nucleotide phosphodiesterase family and PDE7 subfamily,which regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B gene is expressed predominantly in the brain and in a number of other tissues including liver, heart, thyroid glands, and skeletal muscle, but not expressed in blood leukocytes (PMID:12388353).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE7B antibody 17771-1-AP | Download protocol |
| IHC protocol for PDE7B antibody 17771-1-AP | Download protocol |
| IF protocol for PDE7B antibody 17771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurobiol Aging Silencing phosphodiesterase 7B gene by lentiviral-shRNA interference attenuates neurodegeneration and motor deficits in hemiparkinsonian mice.
| ||
Stem Cells Phosphodiesterase7 Inhibition Activates Adult Neurogenesis in Hippocampus and Subventricular Zone in vitro and in vivo. | ||
Stem Cells Transl Med Phosphodiesterase 7 inhibition induces dopaminergic neurogenesis in hemiparkinsonian rats. | ||
BMC Cancer Methylation-regulated tumor suppressor gene PDE7B promotes HCC invasion and metastasis through the PI3K/AKT signaling pathway
|
