Validation Data Gallery

Filter: AllWBIHC

WB analysis of MDA-MB-453s using 55076-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human gliomas using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human thyroid cancer using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 55076-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDGFC Polyclonal antibody

PDGFC Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55076-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MDA-MB-453s cells
Positive IHC detected in	human kidney tissue, human colon tissue, human gliomas tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

55076-1-AP targets PDGFC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	platelet derived growth factor C
Calculated molecular weight	39 kDa
Observed molecular weight	39 kDa
GenBank accession number	NM_016205
Gene Symbol	PDGFC
Gene ID (NCBI)	56034
RRID	AB_2881263
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NRA1
Storage Buffer	0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDGFC is a member of the platelet-derived growth factor family. It is a monomeric protein secreted as a latent dimeric factor. Expression of PDGFC was highest in prostate, testis, and uterus, with weaker expression in spleen, thymus, and small intestine, and little expression in colon and peripheral blood leukocytes.

Protocols

Product Specific Protocols
WB protocol for PDGFC antibody 55076-1-AP	Download protocol
IHC protocol for PDGFC antibody 55076-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Br J Cancer Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis. Authors - Xiang Li View Article
human	WB	Int J Biol Sci Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA. Authors - Meicen Zhou View Article
human	WB	Oral Dis Fibroblast regulates angiogenesis in assembled oral cancer organoid: A possible role of NNMT Authors - Mohammed Holkom View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MDA-MB-453s using 55076-1-AP

IHC staining of human kidney using 55076-1-AP