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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFR beta/CD140b Polyclonal antibody (13449-1-AP)

    WB analysis using 13449-1-AP (same clone as 13449-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13449-1-AP (PDGFRB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using PDGFR beta/CD140b Polyclonal antibody (13449-1-AP)

    IP experiment of mouse lung using 13449-1-AP (same clone as 13449-1-PBS)

    IP result of anti-PDGFR beta (IP:13449-1-AP, 4ug; Detection:13449-1-AP 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta/CD140b Polyclonal antibody (13449-1-AP)

    IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta/CD140b Polyclonal antibody (13449-1-AP)

    IHC staining of human placenta using 13449-1-AP (same clone as 13449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13449-1-AP (PDGFRB antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13449-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDGFR beta/CD140b Polyclonal antibody, PBS Only

PDGFR beta/CD140b Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13449-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13449-1-PBS targets PDGFR beta/CD140b in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4285

Product name: Recombinant human PDGFRB protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 755-1106 aa of BC032224

Sequence: LDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL

相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor receptor, beta polypeptide
Calculated molecular weight 1106 aa, 124 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC032224
Gene Symbol PDGFR beta
Gene ID (NCBI) 5159
RRIDAB_2162644
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP09619
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)

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