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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDGFR beta Recombinant antibody

PDGFR beta Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230214D11

Cat no : 82943-1-RR

Synonyms

PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFR beta Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDGFR beta Recombinant antibody (82943-1-RR)

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDGFR beta Recombinant antibody (82943-1-RR)

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82943-1-RR

    Affinity and Kinetic Characterization of 82943-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82943-1-RR against Human PDGFRB were performed. The affinity constant is 0.668 nM.

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Tested Applications

Positive WB detected inMG-63 cells, HeLa cells, COLO 320 cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82943-1-RR targets PDGFR beta in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor receptor, beta polypeptide
Calculated molecular weight 1106 aa, 124 kDa
Observed molecular weight180 kDa
GenBank accession numberBC032224
Gene symbol PDGFR beta
Gene ID (NCBI) 5159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDGFR beta antibody 82943-1-RRDownload protocol
IHC protocol for PDGFR beta antibody 82943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols