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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

    WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    WB analysis of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    WB analysis of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    WB analysis of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IP experiment of K-562 using 66119-1-Ig (same clone as 66119-1-PBS)

    IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 4ug; Detection:66119-1-Ig 1:10000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human liver using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human liver using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human heart using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IHC staining of human heart using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDH E1 Alpha Monoclonal antibody (66119-1-Ig)

    IF Staining of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66119-1-Ig (PDH E1α antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PDH E1 Alpha Monoclonal antibody, PBS Only

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66119-1-PBS
Clone No.2B3C10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66119-1-PBS targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PDH E1 Alpha fusion protein Ag12556 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2881518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).