• Featured Product
  • KD/KO Validated

PDH E1 Alpha Polyclonal antibody

PDH E1 Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18068-1-AP

Synonyms

PDH E1α, PDHA1, PDHCE1A, PDHA 1, PDHA


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inHeLa cells, HEK293 cells, mouse brain tissue, rat brain tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18068-1-AP targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDH E1 Alpha fusion protein Ag12701 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Calculated molecular weight 43 kDa
Observed molecular weight 39-48 kDa
GenBank accession numberBC002406
Gene symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2162931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Protocols

Product Specific Protocols
WB protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IHC protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IF protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
IP protocol for PDH E1 Alpha antibody 18068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
humanWB

Cell Res

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine

Authors - Mengqi Li
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
  • KO Validated
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

Cell Metab

Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.

Authors - Shawn M Davidson
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and got nice bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:18068-1-AP)
FH

David (Verified Customer) (09-03-2019)

Good detection with 30µg of protein and dilution at 1/1000

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Brain
PDH E1 Alpha Antibody Western Blot, validation (1/1000 dilution) in Brain (Cat no:18068-1-AP)
FH

Svitlana (Verified Customer) (11-02-2017)

Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line).
PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line). (Cat no:18068-1-AP)