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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDHB Monoclonal antibody

PDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7C10

Cat no : 68238-1-Ig

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1, 2A7C10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDHB Monoclonal antibody (68238-1-Ig)

    WB analysis using 68238-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68238-1-Ig (PDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse kidney using 68238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of mouse kidney using 68238-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat stomach using 68238-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PDHB Monoclonal antibody (68238-1-Ig)

    IHC staining of rat stomach using 68238-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68238-1-Ig (PDHB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDHB Monoclonal antibody (68238-1-Ig)

    IF Staining of HeLa using 68238-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDHB antibody (68238-1-Ig, Clone: 2A7C10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse kidney tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68238-1-Ig targets PDHB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDHB fusion protein Ag6857 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) beta
Calculated molecular weight 39 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000439
Gene symbol PDHB
Gene ID (NCBI) 5162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHB (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

Protocols

Product Specific Protocols
WB protocol for PDHB antibody 68238-1-IgDownload protocol
IHC protocol for PDHB antibody 68238-1-IgDownload protocol
IF protocol for PDHB antibody 68238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols