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KD/KO Validated

PDHX Polyclonal antibody

Name: PDHX Polyclonal antibody
Brand: Proteintech
SKU: 10951-1-AP

PDHX Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10951-1-AP

Synonyms

E3 binding protein, E3BP, OPDX, PDHX, PDX1, proX

Validation Data Gallery

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AllWBIPIHC

WB analysis using 10951-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10951-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10951-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 10951-1-AP
IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug.
IHC staining of human colon cancer using 10951-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 10951-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	BxPC-3 cells, HEK-293 cells, SKOV-3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 3 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

10951-1-AP targets PDHX in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDHX fusion protein Ag1389 相同性解析による交差性が予測される生物種
Full Name	pyruvate dehydrogenase complex, component X
Calculated molecular weight	54 kDa
Observed molecular weight	54 kDa
GenBank accession number	BC010389
Gene symbol	PDHX
Gene ID (NCBI)	8050
RRID	AB_2163102
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PDHX antibody 10951-1-AP	Download protocol
IHC protocol for PDHX antibody 10951-1-AP	Download protocol
IP protocol for PDHX antibody 10951-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	J Hazard Mater Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1 Authors - Hao Chen View Article
human	WB,IHC,IF	Cancer Sci Identification of PDHX as a metabolic target for esophageal squamous cell carcinoma. Authors - Jun Inoue KD Validated View Article
mouse	WB	Cell Calcium The Mitochondrial Ca 2+ import complex is altered in ADPKD Authors - Murali K Yanda View Article
	WB	Front Microbiol Singapore Grouper Iridovirus Induces Glucose Metabolism in Infected Cells by Activation of Mammalian Target of Rapamycin Signaling. Authors - Xixi Guo KD Validated View Article
rat	WB	Int J Biol Sci Hypericin maintians PDX1 expression via the Erk pathway and protects islet β-cells against glucotoxicity and lipotoxicity. Authors - Chen Liang View Article
mouse	WB	PLoS One Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3. Authors - Sol Eri Maria EM View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDHX Polyclonal antibody

PDHX Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 10951-1-AP

WB analysis of SKOV-3 using 10951-1-AP

WB analysis of HEK-293 using 10951-1-AP

IP experiment of HEK-293 using 10951-1-AP

IHC staining of human colon cancer using 10951-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1

Identification of PDHX as a metabolic target for esophageal squamous cell carcinoma.

The Mitochondrial Ca 2+ import complex is altered in ADPKD

Singapore Grouper Iridovirus Induces Glucose Metabolism in Infected Cells by Activation of Mammalian Target of Rapamycin Signaling.

Hypericin maintians PDX1 expression via the Erk pathway and protects islet β-cells against glucotoxicity and lipotoxicity.

Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.