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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDI Monoclonal antibody (66422-1-Ig)

    WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PDI Monoclonal antibody (66422-1-Ig)

    WB analysis of HEK-293 using 66422-1-Ig (same clone as 66422-1-PBS)

    WB result of PDI antibody (66422-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PDI Monoclonal antibody (66422-1-Ig)

    WB analysis of rat brain using 66422-1-Ig (same clone as 66422-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDI Monoclonal antibody (66422-1-Ig)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDI Monoclonal antibody (66422-1-Ig)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using PDI Monoclonal antibody (66422-1-Ig)

    IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using PDI Monoclonal antibody (66422-1-Ig)

    IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDI Monoclonal antibody (66422-1-Ig)

    IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2, PDI Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66422-1-PBS. Detection antibody: 66422-3-PBS. Standard:Ag1747. Range: 0.098-1.563 ng/mL.

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  • KD/KO Validated

PDI Monoclonal antibody, PBS Only (Capture)

PDI Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66422-1-PBS
Clone No.2E6A11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66422-1-PBS targets PDI as part of a matched antibody pair:

MP50798-2: 66422-1-PBS capture and 66422-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PDI fusion protein Ag1747 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2881794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07237
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).