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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDI Recombinant antibody, PBS Only

PDI Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K17

Cat no : 80170-1-PBS

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDI Recombinant antibody (80170-1-RR)

    WB analysis using 80170-1-RR (same clone as 80170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDI Recombinant antibody (80170-1-RR)

    IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PDI Recombinant antibody (80170-1-RR)

    IHC staining of human colon using 80170-1-RR (same clone as 80170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80170-1-RR (PDI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDI Recombinant antibody (80170-1-RR)

    IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80170-1-RR

    Affinity and Kinetic Characterization of 80170-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80170-1-PBS targets PDI in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDI fusion protein Ag1747 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene symbol PDI
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.