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ERp57/ERp60 Polyclonal antibody
ERp57/ERp60 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15967-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 31 publications below |
| IHC | See 10 publications below |
| IF | See 12 publications below |
| IP | See 2 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
15967-1-AP targets ERp57/ERp60 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERp57/ERp60 fusion protein Ag8741 相同性解析による交差性が予測される生物種 |
| Full Name | protein disulfide isomerase family A, member 3 |
| Calculated molecular weight | 505 aa, 57 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC014433 |
| Gene symbol | PDIA3 |
| Gene ID (NCBI) | 2923 |
| RRID | AB_2236784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| IHC protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| IF protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| IP protocol for ERp57/ERp60 antibody 15967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Control Release A facile and smart strategy to enhance bone regeneration with efficient vitamin D3 delivery through sterosome technology | ||
Mol Biol Evol Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. | ||
Redox Biol Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress. | ||
EMBO J TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. | ||
Cell Rep Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Prasanna (Verified Customer) (05-07-2018) | Of the four PDIA3/ERp57 antibodies I tried from various companies, this one gave the cleanest and strongest signal by western blot. I was also able to probe multiple blots with the diluted antibody. I will be always come back to this antibody.
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