Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human placenta tissue, human liver tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
15545-1-AP targets PDIR in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDIR fusion protein Ag7901 相同性解析による交差性が予測される生物種 |
| Full Name | protein disulfide isomerase family A, member 5 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC001625 |
| Gene Symbol | PDIR |
| Gene ID (NCBI) | 10954 |
| RRID | AB_2160995 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDIR antibody 15545-1-AP | Download protocol |
| IHC protocol for PDIR antibody 15545-1-AP | Download protocol |
| IP protocol for PDIR antibody 15545-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER. | ||
Oncotarget Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer. | ||
Viruses PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection
| ||
FEBS J Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import. | ||