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KD/KO Validated

PDIA6 Polyclonal antibody

Name: PDIA6 Polyclonal antibody
Brand: Proteintech
SKU: 18233-1-AP

PDIA6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18233-1-AP

Synonyms

Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 18233-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18233-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18233-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18233-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 18233-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 18233-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 18233-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 18233-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18233-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 18233-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 18233-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18233-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18233-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 18233-1-AP
IP result of anti-PDIA6 (IP:18233-1-AP, 4ug; Detection:18233-1-AP 1:3000) with HEK-293 cells lysate 3000ug.
IHC staining of human stomach using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach cancer using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 18233-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 18233-1-AP
Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 18233-1-AP
Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 18233-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human stomach tissue, human spleen tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 19 publications below
IHC	See 2 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

18233-1-AP targets PDIA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDIA6 fusion protein Ag12940 相同性解析による交差性が予測される生物種
Full Name	protein disulfide isomerase family A, member 6
Calculated molecular weight	48 kDa, 54 kDa
Observed molecular weight	48-54 kDa
GenBank accession number	BC001312
Gene symbol	PDIA6
Gene ID (NCBI)	10130
RRID	AB_10805765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Protocols

Product Specific Protocols
WB protocol for PDIA6 antibody 18233-1-AP	Download protocol
IHC protocol for PDIA6 antibody 18233-1-AP	Download protocol
IF protocol for PDIA6 antibody 18233-1-AP	Download protocol
IP protocol for PDIA6 antibody 18233-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors. Authors - Alessandro Carugo View Article
human	WB	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
mouse	WB	J Exp Med Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development. Authors - Jin Huk Choi View Article
human	WB	Nucleic Acids Res Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis. Authors - Neus Mestre-Farràs KD Validated View Article
human	WB	J Immunother Cancer MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells Authors - Yoshihiro Morimoto View Article
human	WB,IF,IP	Antiviral Res Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production Authors - Lauren Panny View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDIA6 Polyclonal antibody

PDIA6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 18233-1-AP

WB analysis of HEK-293 using 18233-1-AP

WB analysis of HEK-293 using 18233-1-AP

WB analysis of mouse kidney using 18233-1-AP

WB analysis of mouse lung using 18233-1-AP

WB analysis of mouse heart using 18233-1-AP

WB analysis of L02 using 18233-1-AP

WB analysis of mouse heart using 18233-1-AP

WB analysis of HeLa using 18233-1-AP

WB analysis of mouse liver using 18233-1-AP

WB analysis of mouse lung using 18233-1-AP

WB analysis of HeLa using 18233-1-AP

WB analysis of mouse kidney using 18233-1-AP

IP experiment of HEK-293 using 18233-1-AP

IHC staining of human stomach using 18233-1-AP

IHC staining of human stomach using 18233-1-AP

IHC staining of human spleen using 18233-1-AP

IHC staining of human spleen using 18233-1-AP

IHC staining of human stomach cancer using 18233-1-AP

IHC staining of human stomach cancer using 18233-1-AP

IF Staining of HeLa using 18233-1-AP

IF Staining of HeLa using 18233-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.

Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.

MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells

Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production