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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDIA6 Monoclonal antibody (66669-1-Ig)

    WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDIA6 Monoclonal antibody (66669-1-Ig)

    IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDIA6 Monoclonal antibody (66669-1-Ig)

    IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDIA6 Monoclonal antibody (66669-1-Ig)

    IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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PDIA6 Monoclonal antibody, PBS Only

PDIA6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66669-1-PBS
Clone No.1G2D4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66669-1-PBS targets PDIA6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDIA6 fusion protein Ag27608 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 6
Calculated molecular weight 48 kDa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC001312
Gene Symbol PDIA6
Gene ID (NCBI) 10130
RRIDAB_2882023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.