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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDILT Polyclonal antibody

PDILT Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26305-1-AP

Synonyms

PDIA7, PDILT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDILT Polyclonal antibody (26305-1-AP)

    WB analysis using 26305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours; mouse brain tissue, mouse heart tissue and mouse lung tissue are negative control.

  • Western Blot (WB) analysis of mouse testis tissue using PDILT Polyclonal antibody (26305-1-AP)

    WB analysis of mouse testis using 26305-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PDILT Polyclonal antibody (26305-1-AP)

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PDILT Polyclonal antibody (26305-1-AP)

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PDILT Polyclonal antibody (26305-1-AP)

    IF Staining of mouse testis using 26305-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PDILT Polyclonal antibody (26305-1-AP)

    IF Staining of mouse testis using 26305-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26305-1-AP targets PDILT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDILT fusion protein Ag23675 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase-like, testis expressed
Observed molecular weight 67 kDa, 75 kDa
GenBank accession numberBC042607
Gene symbol PDILT
Gene ID (NCBI) 204474
RRIDAB_2880472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum (PMID: 15475357). PDILT interacts with CALR3 in the testicular germ cells and plays an indispensable role in the disulfide formation in the ADAM3. The PDILT knockout mice were male infertile, consistent with the previous observations that spermatozoa lacking ADAM3 cannot migrate through the UTJ and cannot bind to the ZP (PMID: 22357757). It can be detected as 67 kDa and 75kDa due to the glycosylation.

Protocols

Product Specific Protocols
WB protocol for PDILT antibody 26305-1-APDownload protocol
IHC protocol for PDILT antibody 26305-1-APDownload protocol
IF protocol for PDILT antibody 26305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols