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  • Featured Product
  • KD/KO Validated

PDIR Polyclonal antibody, PBS Only

PDIR Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29593-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDIR Polyclonal antibody (29593-1-AP)

    WB analysis using 29593-1-AP (same clone as 29593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDIR Polyclonal antibody (29593-1-AP)

    WB analysis using 29593-1-AP (same clone as 29593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDIR Polyclonal antibody (29593-1-AP)

    IHC staining of human placenta using 29593-1-AP (same clone as 29593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDIR Polyclonal antibody (29593-1-AP)

    IHC staining of human placenta using 29593-1-AP (same clone as 29593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29593-1-PBS targets PDIR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDIR fusion protein Ag31479 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 5
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC001625
Gene symbol PDIR
Gene ID (NCBI) 10954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).