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PDK1 Polyclonal antibody, PBS Only

Name: PDK1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 18262-1-PBS

PDK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Review Product

Cat no : 18262-1-PBS

Synonyms

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 18262-1-AP (same clone as 18262-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
WB analysis using 18262-1-AP (same clone as 18262-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
WB analysis using 18262-1-AP (same clone as 18262-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 18262-1-AP (same clone as 18262-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDK1 antibody (18262-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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Cat No. 18262-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18262-1-PBS targets PDK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDK1 fusion protein Ag13121 相同性解析による交差性が予測される生物種
Full Name	pyruvate dehydrogenase kinase, isozyme 1
Calculated molecular weight	436 aa, 49 kDa
Observed molecular weight	46-55 kDa
GenBank accession number	BC039158
Gene symbol	PDK1
Gene ID (NCBI)	5163
RRID	AB_10598310
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDK1 Polyclonal antibody, PBS Only

PDK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Validation Data Gallery

WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

IF Staining of HEK-293 using 18262-1-AP (same clone as 18262-1-PBS)

Tested Applications

Recommended dilution

Product Information

Background Information