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PDK4 Polyclonal antibody

Name: PDK4 Polyclonal antibody
Brand: Proteintech
SKU: 12949-1-AP
Rating: 5.00 (1 reviews)

PDK4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 12949-1-AP

Synonyms

PDHK4, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 12949-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12949-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12949-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 12949-1-AP
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 12949-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12949-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDK4 antibody (12949-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 12949-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, NCI-H1299 cells, mouse heart tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected in	human lung cancer tissue, human pancreas cancer tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 63 publications below
IHC	See 8 publications below
IF	See 9 publications below
ELISA	See 1 publications below

Product Information

12949-1-AP targets PDK4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDK4 fusion protein Ag3629 相同性解析による交差性が予測される生物種
Full Name	pyruvate dehydrogenase kinase, isozyme 4
Calculated molecular weight	411 aa, 46 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC040239
Gene symbol	PDK4
Gene ID (NCBI)	5166
RRID	AB_2161499
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

Protocols

Product Specific Protocols
WB protocol for PDK4 antibody 12949-1-AP	Download protocol
IHC protocol for PDK4 antibody 12949-1-AP	Download protocol
IF protocol for PDK4 antibody 12949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine Authors - Mengqi Li View Article
human	IF	Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. Authors - Li Wang View Article
human	WB	Sci Transl Med Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice. Authors - Biying Zhu View Article
human	WB	Mol Syst Biol STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer. Authors - Monika Oberhuber View Article
human	WB	Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. Authors - Xiaoxin Zhang View Article
mouse	IF	Diabetes Glucose controls glucagon secretion by regulating fatty acid oxidation in pancreatic alpha cells Authors - Sarah L Armour View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Wei (Verified Customer) (01-30-2020)

Strong, sharp band as predicted size around 50kDa. It downregulates in DCM samples.

Applications: Western Blot,
Cell Tissue Type: Mouse Heart

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDK4 Polyclonal antibody

PDK4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 12949-1-AP

WB analysis of mouse heart using 12949-1-AP

WB analysis of mouse skeletal muscle using 12949-1-AP

WB analysis of NCI-H1299 using 12949-1-AP

IHC staining of human lung cancer using 12949-1-AP

IHC staining of human lung cancer using 12949-1-AP

IHC staining of human pancreas cancer using 12949-1-AP

IHC staining of human pancreas cancer using 12949-1-AP

IHC staining of mouse heart using 12949-1-AP

IHC staining of mouse skeletal muscle using 12949-1-AP

IHC staining of rat heart using 12949-1-AP

IF Staining of HepG2 using 12949-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Glucose controls glucagon secretion by regulating fatty acid oxidation in pancreatic alpha cells

Reviews