Featured Product
KD/KO Validated

PDLIM1/CLP36 Polyclonal antibody

Name: PDLIM1/CLP36 Polyclonal antibody
Brand: Proteintech
SKU: 11674-1-AP
Rating: 5.00 (1 reviews)

PDLIM1/CLP36 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11674-1-AP

Synonyms

PDLIM1, hCLIM1, Elfin, C-terminal LIM domain protein 1, CLP-36

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11674-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11674-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of human lung using 11674-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 11674-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 11674-1-AP
IP result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.
IHC staining of human pancreas cancer using 11674-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of MCF-7 using 11674-1-AP
Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of MCF-7 using 11674-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDLIM1/CLP36 antibody (11674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 11674-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C2C12 cells, human lung tissue, human ileum tissue, mouse heart tissue, mouse lung
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

11674-1-AP targets PDLIM1/CLP36 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDLIM1/CLP36 fusion protein Ag2274 相同性解析による交差性が予測される生物種
Full Name	PDZ and LIM domain 1
Calculated molecular weight	329 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC000915
Gene symbol	PDLIM1/CLP36
Gene ID (NCBI)	9124
RRID	AB_2161631
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Protocols

Product Specific Protocols
WB protocol for PDLIM1/CLP36 antibody 11674-1-AP	Download protocol
IHC protocol for PDLIM1/CLP36 antibody 11674-1-AP	Download protocol
IF protocol for PDLIM1/CLP36 antibody 11674-1-AP	Download protocol
IP protocol for PDLIM1/CLP36 antibody 11674-1-AP	Download protocol
FC protocol for PDLIM1/CLP36 antibody 11674-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Autophagy Autophagy is required for ectoplasmic specialization assembly in Sertoli cells. Authors - Chao Liu KD Validated View Article
mouse	WB	Autophagy Autophagy regulates spermatid differentiation via degradation of PDLIM1. Authors - Yongliang Shang View Article
human	WB	EMBO Rep Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB. Authors - Yusha Wang View Article
mouse	WB	Open Biol Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Authors - Shiguo Li View Article
human	WB,IF	Exp Cell Res CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Authors - Miyazaki Kazufumi K KD Validated View Article
human	WB,IHC	Vasc Med Decreased PDLIM1 expression in endothelial cells contributes to the development of intracranial aneurysm Authors - Yan Yan KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Joleen (Verified Customer) (06-03-2019)

PDLIM1 shows up as a clear band at the correct molecular weight.

Applications: Western Blot,
Primary Antibody Dilution: 1:1k
Cell Tissue Type: MDCK GII

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDLIM1/CLP36 Polyclonal antibody

PDLIM1/CLP36 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11674-1-AP

WB analysis of mouse heart using 11674-1-AP

WB analysis of human lung using 11674-1-AP

WB analysis of human ileum using 11674-1-AP

IP experiment of mouse heart using 11674-1-AP

IHC staining of human pancreas cancer using 11674-1-AP

IF Staining of MCF-7 using 11674-1-AP

IF Staining of MCF-7 using 11674-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.

Autophagy regulates spermatid differentiation via degradation of PDLIM1.

Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.

Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.

CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.

Decreased PDLIM1 expression in endothelial cells contributes to the development of intracranial aneurysm

Reviews