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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDLIM3 Polyclonal antibody

PDLIM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13199-2-AP

Synonyms

PDZ and LIM domain 3, Alpha-actinin-2-associated LIM protein, ALP, Actinin-associated LIM protein, Actinin associated LIM protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PDLIM3 Polyclonal antibody (13199-2-AP)

    WB analysis of human heart using 13199-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PDLIM3 Polyclonal antibody (13199-2-AP)

    IHC staining of human stomach cancer using 13199-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13199-2-AP targets PDLIM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM3 fusion protein Ag3934 相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 3
Calculated molecular weight 316 aa, 34 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC027870
Gene symbol PDLIM3
Gene ID (NCBI) 27295
RRIDAB_2161653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDLIM3 antibody 13199-2-APDownload protocol
IHC protocol for PDLIM3 antibody 13199-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Tissue Eng

Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration

Authors - Shijie Fan
View Article