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PDLIM4 Polyclonal antibody

PDLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18543-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, K-562 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18543-1-AP targets PDLIM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM4 fusion protein Ag13424 相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 4
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC016765
Gene Symbol PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3085565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).

Protocols

Product Specific Protocols
WB protocol for PDLIM4 antibody 18543-1-APDownload protocol
IHC protocol for PDLIM4 antibody 18543-1-APDownload protocol
IF protocol for PDLIM4 antibody 18543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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